Agnieszka Swiatecka-Urban M.D., FASN

Assistant Professor Pediatrics

Agnieszka Swiatecka-Urban Assistant Professor of Pediatrics and of Cell Biology A. Personal Statement I have a long-standing research interest in elucidating the regulation of post-maturational trafficking of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) as well as the role of TGF-β1 in the pro-inflammatory responses in CF airway. B. Positions and Honors Positions and Employment 1994-1995 Intern, Pediatrics, St. Vincent’s Hospital, New York, NY 1995-1997 Resident, Pediatrics, St. Vincent’s Hospital, New York, NY 1997-1998 Chief Resident, Pediatrics, St. Vincent’s Hospital, New York, NY 1998-2001 Postdoctoral Fellow, Department of Pediatric, Division of Nephrology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, NY 2001-2003 Postdoctoral Research Fellow, Department of Physiology, Dartmouth Medical School, Hanover, NH 2003-2006 Research Assistant Professor, Department of Physiology, Dartmouth Medical School, Hanover, NH 2007- Assistant Professor of Pediatrics and of Cell Biology & Physiology, Children’s Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 2008- Faculty at the Cell Biology and Molecular Physiology Graduate Program, University of Pittsburgh School of Medicine, Pittsburgh, PA Other Experience and Professional Memberships 1998- The American Society of Nephrology 2006 Moderator at the 20th North American Cystic Fibrosis Conference roundtable sessions: Endo/Exocytic Regulation of CFTR 2007 Co-chair of the Special Group Discussion entitled “Endo/Exocytic Regulation of CFTR” at the European Cystic Fibrosis Society Basic Science Conference in Tavira, Portugal in April, 2007 2008- Member of the Cystic Fibrosis Foundation Research and Research Training Committee 2009- Peer Reviewer for the American Heart Association 2009- Society for Pediatric Research 2010- Member of the Editorial Board of the Physiological Frontiers 2014- Chair, the First National Symposium: Nephrotic Syndrome in Pediatric Patients–Clinical Challenges and Evidence-based Management; Children’s Hospital of Pittsburgh of UPMC Pittsburgh, PA; Honors 2001- Certified, American Board of Pediatrics and Pediatric Nephrology 2005 - Fellow of the American Society of Nephrology 2012 - U.S. Patent# 8,153,597; Issued 4/12/2012. Title: Modulators of the ABC Transporter Family and Method for Their Use C. Selected Peer-reviewed Publications Manuscripts most relevant to the current application 1. Cihil CM, Ellinger P, Fellows A, Beer Stolz D, Madden DR, Swiatecka-Urban A (2012) Dab2 Facilitates AP-2 Independent Recruitment of CFTR to Endocytic Vesicles in Polarized Human Airway Epithelial Cells. J Biol Chem 287(18):15087-99. PMCID:22399289 (Featured at the Global Medical Discovery Series: Key Scientific Articles) 2. Madden DR, Swiatecka-Urban A (2012) Tissue-specific Control of CFTR Endocytosis by Dab2: Cargo Recruitment as a Therapeutic Target. Communicative and Integrative Biology Journal 5(5):1-4. PMCID: 23181163 3. Snodgrass SM, Cihil KM, Cornuet PK, Myerburg MM, Swiatecka-Urban A (2013) TGF-ß1 Inhibits CFTR Biogenesis and Prevents Functional Rescue of ∆F508-CFTR in Primary Differentiated Human Bronchial Epithelial Cells. PLoS One 8(5):e63167. PMCID:23671668 (Featured at the Global Medical Discovery Series: Key Scientific Articles) 4. Cihil K, Swiatecka-Urban A (2013) The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins. J.Vis. Exp, December 13(82). doi: 10.3791/50867. PMCID: 24378656 5. Luz S, Cihil KM, Brautigan DL, Amaral MD, Farinha CM, Swiatecka-Urban A (2014) LMTK2 Mediated Phosphorylation Regulates CFTR Endocytosis in Human Airway Epithelial Cells. J. Biol. Chem. Epub 4/11/14. doi:10.1074/jbc.M1114.563742. PMCID:24727471 Additional publications of importance to the field (in chronological order) 1. Swiatecka-Urban A, Duhaime M, Coutermarsh B, Karlson K, Collawn J, Milewski M, Cutting G, Guggino W, Langford G, Stanton B (2002) PDZ-Domain Interaction Controls the Endocytic Recycling of the Cystic Fibrosis Transmembrane Conductance Regulator. J Biol Chem 277(42):40099-105. PMCID: 12167629 2. Swiatecka-Urban A, Boyd C, Coutermarsh B, Karlson KH, Barnaby R, Aschenbrenner L, Langford GM, Hasson T, Stanton BA (2004) Myosin VI Regulates Endocytosis of the Cystic Fibrosis Transmembrane Conductance Regulator. J Biol Chem 279(36):38025-31. PMCID: 15247260 3. Swiatecka-Urban A, Brown A, Moreau-Marquis S, Renuka BJ, Coutermarsh B, Barnaby R, Karlson KH, Flotte TR, Fukuda M, Langford GM, Stanton BA (2005) The Short Apical Membrane Half-life of Rescued ∆F508-CFTR Results from Accelerated Endocytosis of ∆F508-CFTR in Polarized Human Airway Epithelial Cells. J Biol Chem 280(44):36762-72. PMCID: 16131493 4. Swiatecka-Urban A, Marquis-Moreau S, MacEachran D, Connolly JP, Stanton CR, Su JR, Barnaby R, O’Toole G, Stanton BA (2006) P. aeruginosa Inhibits Endocytic Recycling of ∆F508-CFTR in Polarized Human Airway Epithelial Cells. Am J Physiol Cell Physiol 290(3):C862-72. PMCID: 16236828 5. Swiatecka-Urban A, Talebian L, Kanno E, Moreau-Marquis S, Coutermarsh B, Hansen K, Barnaby R, Karlson KH, Barnaby R, Cheney RE, Langford GM, Fukuda M, Stanton BA (2007) Myosin Vb is Required for Trafficking of CFTR in Rab11a-specific Apical Recycling Endosomes in Polarized Human Airway Epithelial Cells. J Biol Chem 282(32):23725-36. PMCID: 17462998 6. Daniel P. MacEachran, Siying Ye, Jennifer M. Bomberger, Deborah A. Hohan, Agnieszka Swiatecka-Urban, Bruce A. Stanton, George A. O’Toole (2007) The Pseudomonas aeruginosa secreted protein, PA2934, decreases apical membrane expression of the cystic fibrosis transmembrane conductance regulator. Infection and Immunity 75(8):3902-12. PMCID: 17502391 7. Moreau-Marquis S, Bomberger J, Anderson GG, Swiatecka-Urban A, Siying Y, OToole GA, Stanton BA (2008) The ∆F508-CFTR Mutation Results in Increased Biofilm Formation by Pseudomonas aeruginosa by Increasing Iron Availability. Am J Physiol Lung Cell Mol Physiol 295(1):L25-37. PMCID: 18359885 8. Ye S, Cihil K, Beer Stolz D, Pilewski JM, Stanton BA, Swiatecka-Urban A (2010) c-Cbl Facilitates Endocytosis and Lysosomal degradation of CFTR in Human Airway Epithelial Cells. J Biol Chem 285(35):27008-18. PMCID: 20525683 9. Cihil K, Zimnik A, Swiatecka-Urban A (2013) c-Cbl Reduces Stability of Rescued ∆F508-CFTR in Human Airway Epithelial Cells – Implications for Cystic Fibrosis Treatment. Communicative and Integrative Biology Journal 6(2):e23094. PMCID: 23750297


Appearances