Nam Soo Joo Ph.D.

Senior Research Scientist

I have been working in CF research field with a special emphasis on a potential role played by airway submucosal glands in the process of CF lung disease since I joined the cystic fibrosis research laboratory, directed by Dr. Jeffrey J. Wine, at Stanford in 1999. Since then I have contributed to the development of important methods in collaboration with colleagues at Stanford: an in situ optical gland secretion assay for measuring fluid secretion rates and compositions from individual airway submucosal glands; a bioluminescence assay for bacterial killing with saliva samples; proteomic assay of gland mucus from CF & control human airways and a mucociliary clearance (MCC) assay for measuring a mucociliary clearance velocity. With these methodologies, we discovered defects in submucosal gland secretory functions in CF airways from human and CF animal models (CF pigs and CF ferrets). MCC is a critical part of airway innate defense and MCC is impaired in CF subjects and CF animal models. Recently we discovered a synergistic MCC in ferret tracheae and the observation was reproduced in piglet tracheae. To better understand the underlying mechanism of synergistic enhancement of MCC and to render a potential clinical benefit to CF patients, experiments are underway to using MCC assay, Ussing Isc experiment and in situ gland secretion assay with ex vivo animals, including glandless rabbit tracheae.

Appearances