Neeraj Sharma D.V.M., MV.S.c, Ph.D.

Johns Hopkins School of Medicine

I am a molecular biologist with expertise in cystic fibrosis research. As a graduate student at PGIMER, I have identified mutations in the Cystic Fibrosis Conductance Regulator (CFTR) gene responsible for causing cystic fibrosis in Indian population. More than 1700 mutations in the CFTR gene have been reported world-wide, but it is unclear how many of them actually cause the inherited disease. Curious to learn about heterogeneity among these CFTR variants, I joined Dr. Garry Cutting's lab at John Hopkins and contributed towards characterizing the disease liability of CFTR mutations which brings certainty to families about a CF diagnosis. As a PI on a CF Foundation and Gilead Inc. funded grants, I am systematically evaluating nonsense and splice-site mutations to inform treatments. Working under the mentorship of Dr. Cutting on CFTR2 Cell Center project, I have mastered the techniques of creating expression minigenes and inserting them into CF model cell lines to accelerate the design process for new drugs tailored to a particular mutation.